https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43347 Thu 15 Sep 2022 15:25:54 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49337 Thu 11 May 2023 15:21:07 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30895 m increased linearly with increasing arsenate concentration, indicating a decrease in affinity of the enzyme for the substrate. The inhibition constant K_i increased after the ALP was immobilized on montmorillonite and goethite. Thermodynamic properties such as activation energy, activation enthalpy, Gibbs activation free energy and activation entropy increased when arsenate was added, which suggests that more energy is required to form the activated molecule and the enzyme–substrate complex. The reaction was less spontaneous and the enzyme–substrate complex was less ordered. Immobilization of the ALP on minerals reduced the variation in kinetic and thermodynamic properties in the presence of arsenate, indicating that immobilization provided protection from arsenate stress. Arsenate toxicity to soil ALP was affected markedly by soil texture. The results suggest that the type of arsenate inhibition on free soluble ALP was competitive inhibition, whereas for immobilized and soil ALPs, the inhibition was competitive or mixed inhibition depending on the mineral or soil property.]]> Sat 24 Mar 2018 07:30:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26714 Pseudomonas sp., Pseudomonas putida TPHK-1 and Pseudomonas aeruginosa TPHK-4, were more efficient in degrading high concentrations of the hydrocarbons than the other two strains, Stenotrophomonas maltophilia TPHK-2 and Acenitobacter sp. TPHK-3. P. putida TPHK-1 exhibited tolerance to very high concentrations of heavy metals such as cadmium, lead, zinc and copper. The innate ability of P. putida TPHK-1, as evidenced by the amplified genes alkB1 and alkB2 that encode alkane hydroxylases, and cat12o and cat23o coding for catechol dioxygenase, in degrading diesel oil in the presence of heavy metals is far greater than that of the strains reported in the literature. Heavy metal tolerance coupled with rapid degradation of hydrocarbons, even at high concentrations, suggests that P. putida TPHK-1 has a great potential in remediating soils contaminated with mixtures of hydrocarbons and heavy metals.]]> Sat 24 Mar 2018 07:26:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24551 via an assembly of dye-FS-AgNP-GO. Using this assembly, we successfully detected FSs including pentadecafluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), and 1H,1H,2H,2H-perfluorooctanesulfonic acid (6:2FTS), with a limit-of-detection (LOD) of ~50 ppb (~120 nM) for PFOA.]]> Sat 24 Mar 2018 07:11:30 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30571 Mon 23 Sep 2019 10:29:19 AEST ]]>